天麻素分离纯化工艺的研究 -药学专业

摘要

天麻素作为一种重要的药用成分，广泛存在于天麻中，其分离纯化工艺的研究对于提高天麻素的提取效率和纯度具有重要意义。本研究旨在探索一种高效、环保且经济可行的天麻素分离纯化方法，以满足医药行业对高纯度天麻素的需求。通过对比多种传统分离技术，创新性地引入超临界二氧化碳萃取结合大孔树脂吸附法，优化了萃取条件与吸附参数。实验结果表明，在特定压力温度条件下，超临界二氧化碳萃取能显著提高天麻素的得率；而经过筛选的大孔树脂则表现出优异的选择性和再生性能，使得最终产品纯度达到98%以上。该工艺不仅简化了操作流程，降低了生产成本，还有效减少了有机溶剂使用量，符合绿色化学理念。此外，本研究首次建立了基于响应面法的数学模型来预测并优化整个分离过程的关键参数，为实现工业化生产提供了理论依据和技术支持，具有重要的应用价值和广阔的市场前景。

关键词：天麻素; 超临界二氧化碳萃取; 大孔树脂吸附

Abstract

Gastrodin, as an important medicinal component, is widely present in Gastrodia elata. The study of its separation and purification processes is crucial for improving extraction efficiency and purity. This research aims to explore an efficient, environmentally friendly, and economically viable method for gastrodin separation and purification to meet the pharmaceutical industry's demand for high-purity gastrodin. By comparing various traditional separation techniques, this study innovatively introduces supercritical carbon dioxide extraction combined with macroporous resin adsorption, optimizing extraction conditions and adsorption parameters. Experimental results demonstrate that under specific pressure and temperature conditions, supercritical carbon dioxide extraction significantly enhances gastrodin yield. The selected macroporous resin exhibits excellent selectivity and regeneration performance, achieving a final product purity of over 98%. This process not only simplifies operational procedures and reduces production costs but also effectively minimizes the use of organic solvents, aligning with green chemistry principles. Additionally, this study establishes a mathematical model based on response surface methodology for the first time to predict and optimize key parameters of the entire separation process, providing theoretical basis and technical support for industrial production, which holds significant application value and broad market prospects.

Key words:Gastrodin; Supercritical Carbon Dioxide Extraction; Macroporous Resin Adsorption; Response Surface Methodology Optimization; Green Chemical Process

中文摘要 I

英文摘要 II

引言 1

第1章、天麻素提取工艺研究 2

1.1、原料预处理方法 2

1.2、提取溶剂选择 2

1.3、提取条件优化 3

第2章、分离技术筛选与评价 4

2.1、溶剂萃取法研究 4

2.2、色谱分离技术 4

2.3、分离效果评估 5

第3章、纯化工艺开发与优化 6