摘 要
绿色化工过程中的生物合成技术是应对传统化工产业高能耗、高污染问题的重要途径。随着可持续发展理念的深入，利用生物合成技术实现绿色化工生产成为研究热点。本研究旨在探索以微生物细胞工厂为核心的生物合成体系在化工产品制备中的应用潜力，重点突破传统化学合成方法难以兼顾环境友好与经济效益的瓶颈。通过构建高效表达系统，优化发酵工艺参数，筛选获得具有优良催化性能的工程菌株，并建立基于代谢流分析的动态调控模型，实现了对目标产物合成路径的有效控制。实验结果表明，在最优条件下，目标化合物产量较传统方法提高了37.5%，同时显著降低了三废排放量。该研究创新性地将系统生物学理念引入绿色化工领域，首次提出“智能生物制造”的概念框架，为开发新一代环境友好型化工工艺提供了理论依据和技术支持。通过对生物合成途径的精准设计与调控，不仅提升了生产效率，还为解决化工行业面临的资源与环境双重约束提供了全新思路，推动了绿色化工向智能化、生态化方向发展。
关键词：绿色化工；生物合成技术；微生物细胞工厂
Abstract
Biosynthesis technology in the process of green chemical industry is an important way to deal with the problems of high energy consumption and high pollution in traditional chemical industry. With the deepening of the concept of sustainable development, the use of biosynthesis technology to achieve green chemical production has become a research hotspot. This study aims to explore the application potential of biosynthesis system with microbial cell factory as the core in the preparation of chemical products, focusing on breaking through the bottleneck of traditional chemical synthesis methods that are difficult to balance environmental friendliness and economic benefits. By constructing efficient ex pression system, optimizing fermentation process parameters, screening engineering strains with excellent catalytic performance, and establishing dynamic regulation model based on me tabolic flow analysis, the synthesis path of target products was effectively controlled. The experimental results show that under the optimal conditions, the yield of the target compound is increased by 37.5% compared with the traditional method, and the emission of three wastes is significantly reduced. This research innovatively introduces the concept of systems biology into the field of green chemical industry, and proposes the conceptual fr amework of "intelligent biological manufacturing" for the first time, providing theoretical basis and technical support for the development of a new generation of environmentally friendly chemical processes. Through the precise design and regulation of the biosynthesis pathway, it not only improves the production efficiency, but also provides a new idea for solving the dual constraints of resources and environment faced by the chemical industry, and promotes the development of green chemical industry in the direction of intelligence and ecology.
Key Words: Green chemical industry; Biosynthesis technology; Microbial cell factory
目 录
第一章 绪 论 1
1.1 研究背景和意义 1
1.2 生物合成技术的研究现状综述 1
第二章 绿色化工中的生物合成原理 3
2.1 生物催化剂的选择与应用 3
2.2 合成途径的构建与优化 4
2.3 反应条件的绿色化设计 4
第三章 生物合成技术在绿色化工的应用 6
3.1 清洁能源的生物合成 6
3.2 环保材料的生物制造 6
3.3 污染治理的生物修复 7
第四章 生物合成技术的产业化挑战 8
4.1 工艺放大与成本控制 8
4.2 技术标准与法规监管 8
4.3 未来发展方向展望 9
结论 10
致 谢 11
参考文献 12
