蓝光对视网膜细胞损伤机制及防护策略研究
摘 要
随着电子设备的普及，蓝光暴露已成为威胁视网膜健康的重要环境因素。本研究旨在探讨蓝光对视网膜色素上皮细胞的损伤机制，并开发有效的防护策略。通过建立体外细胞模型，采用流式细胞术、荧光探针和Western blot等技术，系统评估了不同强度蓝光照射下细胞的凋亡率、活性氧水平和线粒体膜电位变化。研究发现，蓝光可诱导细胞内活性氧显著升高，导致线粒体功能障碍和细胞凋亡关键蛋白表达异常。在此基础上，创新性地筛选出具有显著抗氧化作用的天然化合物X，证实其可通过调控Nrf2/ARE信号通路有效减轻蓝光诱导的氧化应激损伤。进一步通过分子对接模拟揭示了化合物X与Keap1蛋白的特异性结合位点。
关 键 词：蓝光损伤；视网膜色素上皮细胞；氧化应激
Study on the mechanism and protection strategy of retinal cell damage caused by blue light
ABSTRACT
With the popularity of electronic devices, blue light exposure has become an important environmental factor threatening to retinal health. The aim of this study was to investigate the mechanism of blue light damage to retinal pigment epithelial cells and to develop effective protective strategies. The rate of in vitro cell model, levels of reactive oxygen species and mitochondrial membrane potential changes of cells with different intensity of blue light were systematically evaluated using flow cytometry, fluorescent probes and Western blot. It was found that blue light could induce a significant increase in intracellular reactive oxygen species, leading to mitochondrial dysfunction and abnormal ex pression of key proteins in apoptosis. Based on this result, the natural compound X with significant antioxidant effect was innovatively screened and confirmed that it could effectively reduce blue light-induced oxidative stress damage by regulating the Nrf 2 / ARE signaling pathway. Further, the specific binding sites of compound X to the Keap 1 protein were revealed by molecular docking simulations.
Key words：Injury to blue light; retinal pigment epithelial cells; oxidative stress
摘 要 I
ABSTRACT II
一、绪论 1
（一）研究背景 1
（二）研究现状 1
二、蓝光对视网膜细胞的损伤机制 2
（一）蓝光的物理特性及其生物学效应 2
（二）视网膜细胞的光感受器损伤机制 2
（三）蓝光诱导的氧化应激与细胞凋亡途径 2
三、蓝光防护材料的筛选与评价 4
（一）蓝光防护材料的筛选标准与方法 4
（二）常见蓝光防护材料的性能比较 4
（三）新型纳米材料在蓝光防护中的应用前景 5
四、基于生物节律的蓝光防护策略 6
（一）昼夜节律与视网膜功能的关系 6
（二）时间依赖性蓝光暴露的生物学效应 6
（三）个性化蓝光防护方案的制定与应用 7
五、结语 8
参考文献 9
谢辞 10
摘 要
随着电子设备的普及，蓝光暴露已成为威胁视网膜健康的重要环境因素。本研究旨在探讨蓝光对视网膜色素上皮细胞的损伤机制，并开发有效的防护策略。通过建立体外细胞模型，采用流式细胞术、荧光探针和Western blot等技术，系统评估了不同强度蓝光照射下细胞的凋亡率、活性氧水平和线粒体膜电位变化。研究发现，蓝光可诱导细胞内活性氧显著升高，导致线粒体功能障碍和细胞凋亡关键蛋白表达异常。在此基础上，创新性地筛选出具有显著抗氧化作用的天然化合物X，证实其可通过调控Nrf2/ARE信号通路有效减轻蓝光诱导的氧化应激损伤。进一步通过分子对接模拟揭示了化合物X与Keap1蛋白的特异性结合位点。
关 键 词：蓝光损伤；视网膜色素上皮细胞；氧化应激
Study on the mechanism and protection strategy of retinal cell damage caused by blue light
ABSTRACT
With the popularity of electronic devices, blue light exposure has become an important environmental factor threatening to retinal health. The aim of this study was to investigate the mechanism of blue light damage to retinal pigment epithelial cells and to develop effective protective strategies. The rate of in vitro cell model, levels of reactive oxygen species and mitochondrial membrane potential changes of cells with different intensity of blue light were systematically evaluated using flow cytometry, fluorescent probes and Western blot. It was found that blue light could induce a significant increase in intracellular reactive oxygen species, leading to mitochondrial dysfunction and abnormal ex pression of key proteins in apoptosis. Based on this result, the natural compound X with significant antioxidant effect was innovatively screened and confirmed that it could effectively reduce blue light-induced oxidative stress damage by regulating the Nrf 2 / ARE signaling pathway. Further, the specific binding sites of compound X to the Keap 1 protein were revealed by molecular docking simulations.
Key words：Injury to blue light; retinal pigment epithelial cells; oxidative stress
摘 要 I
ABSTRACT II
一、绪论 1
（一）研究背景 1
（二）研究现状 1
二、蓝光对视网膜细胞的损伤机制 2
（一）蓝光的物理特性及其生物学效应 2
（二）视网膜细胞的光感受器损伤机制 2
（三）蓝光诱导的氧化应激与细胞凋亡途径 2
三、蓝光防护材料的筛选与评价 4
（一）蓝光防护材料的筛选标准与方法 4
（二）常见蓝光防护材料的性能比较 4
（三）新型纳米材料在蓝光防护中的应用前景 5
四、基于生物节律的蓝光防护策略 6
（一）昼夜节律与视网膜功能的关系 6
（二）时间依赖性蓝光暴露的生物学效应 6
（三）个性化蓝光防护方案的制定与应用 7
五、结语 8
参考文献 9
谢辞 10
